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ECCITE-seq

^ http://purl.obolibrary.org/obo/EFO_0700014


The collective capabilities of CITE-seq and Cell Hashing adapted for compatibility with the 5P / V(D)J single cell kit from 10x Genomics, to allow researchers to perform sample multiplexing, doublet detection and protein detection together with 5’ gene expression and V(D)J reconstruction. [ https://cite-seq.com/eccite-seq/ https://orcid.org/0000-0002-7431-4139 ]

Term info

comment

Through the addition of a custom RT primer and additional PCR reactions on the low molecular weight cDNA fraction, sgRNA capture and single cell CRISPR screens with multimodal readout are made possible.

date

2023-01-12T08:31:22Z

definition

The collective capabilities of CITE-seq and Cell Hashing adapted for compatibility with the 5P / V(D)J single cell kit from 10x Genomics, to allow researchers to perform sample multiplexing, doublet detection and protein detection together with 5’ gene expression and V(D)J reconstruction.

has exact synonym

expanded CRISPR-compatible cellular indexing of transcriptomes and epitopes by sequencing, expanded CRISPR-compatible CITE-seq

Term relations

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