ECCITE-seq
http://purl.obolibrary.org/obo/EFO_0700014
The collective capabilities of CITE-seq and Cell Hashing adapted for compatibility with the 5P / V(D)J single cell kit from 10x Genomics, to allow researchers to perform sample multiplexing, doublet detection and protein detection together with 5’ gene expression and V(D)J reconstruction. [ https://cite-seq.com/eccite-seq/ https://orcid.org/0000-0002-7431-4139 ]
Term info
Through the addition of a custom RT primer and additional PCR reactions on the low molecular weight cDNA fraction, sgRNA capture and single cell CRISPR screens with multimodal readout are made possible.
2023-01-12T08:31:22Z
The collective capabilities of CITE-seq and Cell Hashing adapted for compatibility with the 5P / V(D)J single cell kit from 10x Genomics, to allow researchers to perform sample multiplexing, doublet detection and protein detection together with 5’ gene expression and V(D)J reconstruction.
expanded CRISPR-compatible cellular indexing of transcriptomes and epitopes by sequencing, expanded CRISPR-compatible CITE-seq