Helicos sequencing assay

true single molecule sequencing

url:https://en.wikipedia.org/wiki/Helicos_single_molecule_fluorescent_sequencing

Helicos sequencing is a DNA sequencing which allows sequence identification of billions of DNA molecules immobilized to a surface by using DNA polymerase and fluorescently labeled nucleotides added one at a time. The sequencing process does not requires amplification step and is typically able to produce reads of 25 base pair length., A DNA sequencing by synthesis assay that identifiies the sequnece of billions of DNA molecules immobilized to a surface using DNA polymerase and fluorescently labeled nucleotides added one at a time. The sequencing process does not require an amplification step and is typically able to produce reads of 25 base pair length.

PMID: 18388294. Single-molecule DNA sequencing of a viral genome.

http://purl.obolibrary.org/obo/IAO_0000120

Philippe Rocca-Serra