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immunoprecipitation assay

^ http://purl.obolibrary.org/obo/OBI_0001700


Term info

IAO 0000111

immunoprecipitation assay

definition

An analyte assay in which an input material is mixed with antibodies and bound antigen:antibody complexes are separated out using immunoprecipitation. Either the antibody has known specificy, and the antigen mixture is tested for the presence of a specific antigen, or the antigen solution is well defined and the antibody solution is tested for the presence of antigen specific antibodies.

example of usage

Determining if a cell is producing a protein using a protein specific antibody to immunoprecipitate the cell lysate. Determining if the serum of a patient contains antibodies against HBV core protein by immunoprecipitating purified HBV core protein with the patients serum.

has curation status

http://purl.obolibrary.org/obo/IAO_0000120

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