FISSEQ
http://www.ebi.ac.uk/efo/EFO_0008990
Term info
comment
To support FISSEQ being a child of 'in situ sequencing': this would mirror the layout for 'in-situ hybridization assay'. However, it is not entirely clear if all FISSEQ assays involve a padlock probe, but rolling-circle amplification is applicable
definition
genome-wide profiling of gene expression in situ in fixed cells and tissues, in which RNA is converted into cross-linked cDNA amplicons and sequenced manually on a confocal microscope
has exact synonym
fluorescent in situ RNA sequencing, fluorescent in situ sequencing
term editor
Dani Welter