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Nx1-seq

^ http://www.ebi.ac.uk/efo/EFO_0030021


Next generation one-cell sequencing (Nx1-seq) is a single cell transcriptomic library preparation method which conjugates polystyrene beads to barcode nucleotides by means of emulsion PCR using oligo-dTs. The beads are mixed on a chamber slide containing PDMS microwells which fit one cell per well. The slide is then washed with a cell suspension to capture and barcode individual cells. [ http://www.ncbi.nlm.nih.gov/pubmed/30968360 https://orcid.org/0000-0002-7431-4139 ]

Term info

Subsets

added_for_HCA

creator

paola

definition

Next generation one-cell sequencing (Nx1-seq) is a single cell transcriptomic library preparation method which conjugates polystyrene beads to barcode nucleotides by means of emulsion PCR using oligo-dTs. The beads are mixed on a chamber slide containing PDMS microwells which fit one cell per well. The slide is then washed with a cell suspension to capture and barcode individual cells.

has exact synonym

next generation one-cell sequencing

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