Nx1-seq
http://www.ebi.ac.uk/efo/EFO_0030021
Next generation one-cell sequencing (Nx1-seq) is a single cell transcriptomic library preparation method which conjugates polystyrene beads to barcode nucleotides by means of emulsion PCR using oligo-dTs. The beads are mixed on a chamber slide containing PDMS microwells which fit one cell per well. The slide is then washed with a cell suspension to capture and barcode individual cells. [ http://www.ncbi.nlm.nih.gov/pubmed/30968360 https://orcid.org/0000-0002-7431-4139 ]
Term info
added_for_HCA
paola
Next generation one-cell sequencing (Nx1-seq) is a single cell transcriptomic library preparation method which conjugates polystyrene beads to barcode nucleotides by means of emulsion PCR using oligo-dTs. The beads are mixed on a chamber slide containing PDMS microwells which fit one cell per well. The slide is then washed with a cell suspension to capture and barcode individual cells.
next generation one-cell sequencing