More sensitive version of the Slide-seq method for transferring RNA from tissue sections onto a surface covered in DNA-barcoded beads with known positions, which differs from Slide-seq in the use of a monobase encoding scheme with sequencing by ligation using sequential interrogation by offset primer, and improved parameters for split-pool synthesis of barcoded beads. [ https://www.biorxiv.org/content/10.1101/2020.03.12.989806v1 http://orcid.org/0000-0001-8134-3037 ]