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Slide-seqV2

^ http://www.ebi.ac.uk/efo/EFO_0030062


More sensitive version of the Slide-seq method for transferring RNA from tissue sections onto a surface covered in DNA-barcoded beads with known positions, which differs from Slide-seq in the use of a monobase encoding scheme with sequencing by ligation using sequential interrogation by offset primer, and improved parameters for split-pool synthesis of barcoded beads. [ https://www.biorxiv.org/content/10.1101/2020.03.12.989806v1 http://orcid.org/0000-0001-8134-3037 ]

Term info

creator

paola

date

2021-12-16T08:53:05Z

definition

More sensitive version of the Slide-seq method for transferring RNA from tissue sections onto a surface covered in DNA-barcoded beads with known positions, which differs from Slide-seq in the use of a monobase encoding scheme with sequencing by ligation using sequential interrogation by offset primer, and improved parameters for split-pool synthesis of barcoded beads.