A developmental process, independent of morphogenetic (shape) change, that is required for the midbrain-hindbrain boundary to attain its fully functional state. The midbrain-hindbrain domain of the embryonic brain is comprised of the mesencephalic vesicle and the first rhombencephalic vesicle at early somitogenesis stages. An organizing center at the boundary patterns the midbrain and hindbrain primordia of the neural plate. [ GOC:dgh GOC:cls http://purl.obolibrary.org/obo/go/references/0000021 GOC:dph http://www.ncbi.nlm.nih.gov/pubmed/15541513 GOC:jid ]